Proteomics report (Case study)

1. We use a mix of six proteins to calibrate the instrument on a weekly basis. Using file LW_6mix_4_200fmol.pkl and searching in the Swissprot database on a QTOF instrument find the six proteins used in the calibration and locate the information for each as requested.

 

1. What is the threshold score? ­­___________________________________________ (1 mark)

 

b:                                                                                                                                                            (2 marks each)

Protein:
Species:
Sequence coverage:
Protein score:
Protein Mass:
Calculated pI:
# peptides used in identification:

 

Protein:
Species:
Sequence coverage:
Protein score:
Protein Mass:
Calculated pI:
# peptides used in identification:

 

Protein:
Species:
Sequence coverage:
Protein score:
Protein Mass:
Calculated pI:
# peptides used in identification:

 

Protein:
Species:
Sequence coverage:
Protein score:
Protein Mass:
Calculated pI:
# peptides used in identification:

 

 

 

 

Protein:
Species:
Sequence coverage:
Protein score:
Protein Mass:
Calculated pI:
# peptides used in identification:

 

Protein:
Species:
Sequence coverage:
Protein score:
Protein Mass:
Calculated pI:
# peptides used in identification:

 

1. What is your justification for selecting these 6 proteins?                                                           (2 marks)

 

 

 

 

 

2. Ten proteins were found in a quantitative proteomic exploration of oral biofilms from healthy individuals and individuals with either gingivitis or periodontitis. Using the data provided draw a hive plot and find the answers to the following questions. You will need to build your own node and links files from the data provided.

Axes a = health (0/360°), b=gingivitis (120°), c=periodontitis (240°)

1. Hive plot:                                                                                                                        (3 marks)

(insert here)

 

1. Which protein is most abundant in gingivitis?                                                 (4 marks)

 

From what species does it come?

 

What is its biological function?

 

What mass does it have?

 

1. Which protein is most abundant in periodontitis?                                         (4 marks)

 

From what species does it come?

 

What is its biological function?

 

What mass does it have?

 

1. Which species is most represented in the group of ten found?                              (1 mark)

 

1. To which Socransky complex does it belong?                                                 (1 mark)

 

1. What might the presence of this bacterium suggest?                                  (2 marks)

 

Is this the question you were looking for? If so, place your order here to get started!