Protein Purification:
Protein Purification:
when you receive the pdf file keep in mine the following to help you complete the assignment:
For table 2:32
The dilution ratios and volumes used in column B are as follow:
A–>1:150 (5.3:794.7)
B–>1:70 (11.3:788.7)
C–>1:30 (25.8:774.2)
D–>1:150 (5.3:794.7)
E–>1:390 (2.1:797.9)
And you need to recalculate the df (total)
Then you have to fill in the tables and answer the data analysis questions.
Wavelength used: 595 nm
Samples:
– àBlank 0.2557 Abs
– àSample 1 0.3184 Abs
– àSample 2 0.3317 Abs
– àSample 3 0.3808 Abs
– àSample 4 0.4586 Abs
– àSample 5 0.5264 Abs
– àSample 6 0.5902 Abs
– àSample A 0.0441 Abs
– àSample B 0.0428 Abs
– àSample C 0.0454 Abs
– àSample D 0.0461 Abs
– àSample E 0.4332 Abs
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