Biology Workshop paper to describe basic medium components and concentrations. Describe the contribution/role of each medium component in cell culture.

Workshop 1 – LQB595 (4°/o of final grade)

 

Name: Student number:

 

 

 

Workshop, in Groups of 4.  From lecture worksheets, describe basic medium components and concentrations. Describe the contribution/role of each medium component in cell culture.

 

From lecture worksheets, determine the quantity of cells, oxygen, glucose required to generate a cubic centimeter of tissue.

 

From lecture worksheets, describe integrin and extracellular matrix interactions. Contrast this with cytokine/growth factor signaling interactions.

 

 

 

 

 

 

 

Questions:

 

 

 

1. Calculate oxygen at bottom of a culture plate.

 

 

 

2. At what pH is cell culture medium maintained and how?

 

 

 

3. How can oxygen be toxic?  How is this controlled?

 

 

 

 

 

4. How are “non water soluble” vitamins usually delivered? How are lipids delivered into  medium?

 

 

 

 

 

5. When serum is removed, what “types” proteins need to be replaced or compensated for?

 

 

 

 

 

6. What is adaption to serum-free medium mean and why do we need to think about this? What are the implications?

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